South Ayrshire and Galloway police are taking part in the campaign, which was launched on Monday.
Officers from both areas will work alongside specialist road policing teams, as they carry out dedicated high visibility mobile patrols on the A77 between Ayr and Stranraer.
The initiative comes in response to concerns raised by members of the public, community councils and local councillors and further highlighted by fatalities that have occurred in recent weeks.
The aim of the operation will be to influence driver behaviour through targeted enforcement and awareness.
There will also be Days of Action, which will include road checks at strategic locations in Galloway and Carrick.
Galloway Area Commander Chief Inspector Mark Hollis said: “This initiative is just the first step in an enhanced relationship with our colleagues in South Ayrshire and Ayrshire road policing to ensure a sustainable approach to improving road safety and reducing fatalities on our roads.”
The initiative will run for two weeks.