The travel publication revealed that the region, which covers Dumfries and Galloway and the Scottish Borders, is one of only two UK destinations and 30 worldwide to make the annual list.
The South of Scotland has been named in the Learn category, alongside Manchester, New Mexico, Dresden, El Salvador and Marseille. The region’s history and culture make it the ideal place for delving into the past to learn more about iconic figures such as Robert Burns and Sir Walter Scott.
Melanie Allen, interim chief executive of the South of Scotland Destination Alliance, said: “To be included in the guide is a momentous accolade for the south of Scotland.This news will be an enormous boost to our tourism and hospitality sectors.”