Athletic have previously been a thorn in the Hamilton side, winning the last three meetings.
Who can forget when the Black and Golds enjoyed a thumping 4-1 victory in the fourth round of the Scottish Cup in 2016, at a time when The Accies were a Premiership outfit.
And since becoming manager Peter Murphy has led his side to two League Cup victories over the South Lanarkshire outfit.
Tonight, Murphy’s outfit make a return to the scene of their play-off promotion glory as they travel to New Douglas Park under the floodlights.
Four months have passed since that historic night against Clyde and now the Black and Golds are seven games into life in the third tier, unbeaten in their last four outings.
Last Saturday Peter Murphy’s men drew 1-1 with Alloa at Galabank after Aidan Smith’s late equaliser salvaged a point in a game the boss believes his side could have won.
Murphy said: “I’m pleased that we’re unbeaten in the last four, but I feel like we could have got better results in a couple of the games, Alloa especially.
“We had a very comfortable game and we conceded a real sloppy goal. We finished really strongly and just didn’t take our chances.
“We’ve now got our next three games against full-time teams, so that’s going to be very tough.
“I think the confidence in the players has changed, and their mentality, since the start of the season, and getting a few players back from injury and loan signings in has given us competition for places.”
Hamilton pose a different test, they are unbeaten following relegation from the Championship with six wins and one draw.
Murphy added: “We will go and play our own game and go and try to enjoy the game.
“But we recognise that they are unbeaten, they are a very strong side, they’re a full-time team and there’s a lot expected of them.
“We’ve just got to make sure that we’re disciplined and keep doing what we do.”
The Galabank chief also spoke about the key differences he has noticed following the step-up to League One.
Murphy said: “I think there is more intensity over the 90 minutes in League One where you do have to make changes probably at 60 or 70 minutes just to keep players fresh, that’s the big change.
“And opposition players maybe don’t need as many opportunities to score a goal.”
Murphy also gave an update on the injury front, he added: “Tam Muir trained on Tuesday night which was great, we’ll just see if there’s any reaction from him, Josh Dixon has a little bit of a swollen knee, so we’re trying to manage him, and Steven Swinglehurst is out for a longer period, he’s waiting for an operation in October.”