And Salim Rettie, 35, also faces a charge of sexually assaulting a woman at the coach park at Annandale Water Services at Johnstonebridge.
Rettie from Coatbridge, on Monday pleaded not guilty to committing a breach of the peace on the bus between Glasgow and Johnstonebridge, acting aggressively, challenging other passengers to fight and making threats of violence towards them, as well as repeatedly punching the roof of the bus.
He also denied repeatedly attempting to cuddle a woman at the car park and repeatedly attempting to touch her on the buttocks, behaving in a threatening or abusive manner towards the driver and police.
In addition, he denied being in possession of cannabis.
Trial at Dumfries Sheriff Court has been set for November.