The club announced that a member of their backroom staff has returned a positive Covid-19 test result.
As a result, he whole coaching team have to self-isolate under Scottish government guidelines.
Senior players Willie Gibson and Stephen Dobbie will take temporary charge for Saturday’s Championship match at Somerset Park – which is currently still going ahead as things stand.
A Queens spokesman said: “Following our most recent round of routine testing, we can confirm that a member of our backroom staff has returned a positive COVID-19 test result.
“Therefore, in line with Scottish Government guidelines our whole coaching team are now in self-isolation and will not be at our opening league game away at Ayr tomorrow, which is currently still on as things stand.
“Everyone at the club wishes those involved a speedy recovery and we look forward to welcoming everyone back once it is safe to do so.
“Due to patient confidentiality we will not be making any further comment.”